Probing Structural Perturbation of Biomolecules by Extracting Cryo-EM Data Heterogeneity
Single-particle cryogenic electron microscopy (cryo-EM) has become an indispensable tool to probe high-resolution structural detail of biomolecules.It enables direct visualization of the biomolecules and opens a possibility for averaging molecular images to reconstruct a three-dimensional Coulomb potential density map.Newly developed algorithms for